あね
older sister
A woman at the market, already running the stall on her own while you were still small enough to be hanging onto it. The same nay あね as お姉さん, with the politeness taken off: あね.
Your own older sister. Someone else's is お姉さん. And you'd use that to her face, not あね.
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Emaki is not affiliated with, or endorsed by, Kaishi 1.5k or its authors. The word, reading and meaning come from the Kaishi 1.5k deck, used with their permission. The mnemonic, the note and the kanji breakdown are original work, published under CC BY-SA 4.0.